Recipe

Blocking solution for rabbit

DEPC-PBS (10X), diluted to 1X

Normal goat serum (NGS) (1%, v/v)

Triton X-100 (0.1%, v/v)

Complement-deactivate NGS by incubation for 1 h at 60°C. Store 2-mL aliquots of NGS for up to 12 mo at -20°C.

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  1. doi:10.1101/pdb.rec12082 Cold Spring Harb Protoc 2010: pdb.rec12082-
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