Recipe

Pasteur pipettes with customized opening diameters

MATERIALS

Equipment

Alcohol burner, glass

Pasteur pipettes (glass, long)

Long pipettes usually have a longer conical part than the short variety.

Pencil (diamond-point)

Plastic card (e.g., credit card)

Protective eyegear and gloves

METHOD

This method produces the desired opening size by defined fracturing.

Protect your eyes and hands against flying glass splinters.

  • 1. Hold the pipette horizontally.

  • 2. Make a vertical notch at the conical part of the pipette with a diamond pen.

    Vary the position of the notch to get different diameters in the resulting opening.

  • 3. Use a solid ridge (e.g., a stiff plastic card such as a credit card) to crack the pipette in two.

    With some practice, a vertical cut end is obtained at the intended level of the pipette conus.

  • 4. Flame-polish the sharp edge of the pipette using an alcohol burner.

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  1. doi:10.1101/pdb.rec12084 Cold Spring Harb Protoc 2010: pdb.rec12084-

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