Recipe

Freezing diluent

88 mM citric acid (Sigma C2404)

47 mM D-(+)-glucose (Sigma G6152)

3.5 M DMSO (dimethyl sulfoxide; Sigma D2650)

0.1 M sucrose (Sigma S1888)

0.25 M Tris base (Trizma base; Sigma T6066)

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  1. doi:10.1101/pdb.rec12099 Cold Spring Harb Protoc 2010: pdb.rec12099-
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