Recipe

Paraformaldehyde fixation solution (4%)

Reagent Amount to add Final concentration
Paraformaldehyde stock solution (16%) 50 mL 4%
DEPC-PBS (10X), diluted to 1X 150 mL 0.75X
Add the paraformaldehyde stock solution to 130 mL of 1X DEPC-PBS while stirring. If necessary, adjust the pH to 7.4. Add 1X DEPC-PBS to a final volume of 200 mL. Store aliquots (5-, 10-, or 20-mL) for up to 1 yr at -20°C.
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  1. doi:10.1101/pdb.rec12104 Cold Spring Harb Protoc 2010: pdb.rec12104-
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