| Levamisole (1 M)
|
0.8 mL |
2 mM |
| MgCl2 (1 M)
|
20 mL |
50 mM |
| NaCl (5 M) |
8 mL |
100 mM |
| Tris-Cl (1 M)
|
40 mL |
100 mM |
| Tween 20 |
0.4 mL |
0.1% |
| DEPC-H2O
|
to 400 mL |
|
|
| Add all reagents to ~300 mL DEPC-H2O. Mix. Adjust the volume to 400 mL with DEPC-H2O. Store NTMT for no more than several days at room temperature.
|