Recipe

PBT for rabbit

MATERIALS

Reagents

DEPC-PBS (10X)

DEPC-H2O

Tween 20

METHOD

  • 1. Dilute the 10X DEPC-PBS to 1X with DEPC-H2O.

  • 2. Add 1 mL of Tween 20 to 999 mL of 1X DEPC-PBS.

  • 3. Mix until the Tween 20 is completely dissolved.

  • 4. Store at room temperature for up to 12 mo.

    The final concentration of Tween 20 is 0.1%.

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  1. doi:10.1101/pdb.rec12111 Cold Spring Harb Protoc 2010: pdb.rec12111-

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