Recipe

APBS

25 mL H2O (sterile; Merck)

100 mL PBS (phosphate-buffered saline; Invitrogen)

In this recipe, PBS is adjusted to amphibian osmolarity (225 ± 5 mOsm/L) by the addition of H2O.

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  1. doi:10.1101/pdb.rec12112 Cold Spring Harb Protoc 2010: pdb.rec12112-
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