AEC buffer

AEC tablets (aminoethylcarbazole, 3-amino-9-ethylcarbazole; 20 mg) (Sigma)

DMF (N,N-dimethylformamide) (Fisher Scientific)

Acetic acid (0.1N)

Sodium acetate (0.1M)

Dissolve one AEC tablet (20 mg) in 2 mL of DMF. Add this solution to a final concentration of 0.03% to a 1:2.3 solution of 0.1N acetic acid and 0.1M sodium acetate.

Prepare AEC solution within minutes of use.