Blocking buffer for ELISPOT assay
1X PBS (10X PBS [Roche] diluted to 1X with water)
10 g/L bovine serum albumin (BSA) (Sigma)
Filter through a 22-μM filter (Millipore).
1X PBS (10X PBS [Roche] diluted to 1X with water)
10 g/L bovine serum albumin (BSA) (Sigma)
Filter through a 22-μM filter (Millipore).