Detection buffer for ELISPOT assay
1X PBS (10X PBS [Roche] diluted to 1X with H2O)
0.05% Tween 20
10 g/L bovine serum albumin (BSA)
0.5 μg/mL fluorescein isothiocyanate (FITC)-conjugated anti-IFN-γ monoclonal antibody (mAb) (Mabtech)
0.5 μg/mL biotin-conjugated anti-IL-2 mAb (Becton Dickinson/Pharmingen)
Detection buffer should be prepared within a few hours of use.
