Recipe

R20 medium

RPMI medium 1640 (GIBCO/Invitrogen)

20% fetal bovine serum (FBS)

100 U/mL of penicillin (Multicell)

100 μg/mL streptavidin (Multicell)

2 mM L-glutamine (Multicell)

20 mM HEPES (Sigma)

Adjust pH to 7.0.

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  1. doi:10.1101/pdb.rec12130 Cold Spring Harb Protoc 2010: pdb.rec12130-
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