Recipe

Vector Blue solution

0.1M Tris-Cl (pH 8.2)

0.75% Vector Blue Reagent 1

0.8% Vector Blue Reagent 2

0.5% Vector Blue Reagent 3

Vector Blue Reagents 1, 2, and 3 are from the Vector Blue Alkaline Phosphatase Substrate Kit III (Vector Laboratories). Vector Blue solution should be prepared within hours of use.

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  1. doi:10.1101/pdb.rec12131 Cold Spring Harb Protoc 2010: pdb.rec12131-

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