Recipe

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MATERIALS

Reagents

Chromium potassium sulfate

Gelatin (Sigma)

Haemo-sol (warm)

Equipment

Hot plate

Slide racks

Slides

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METHOD

  • 1. Dissolve 5 g of gelatin and 0.5 g of chromium potassium sulfate in 500 mL of hot (80ºC) H2O. Stir on a hot plate until dissolved (~2 h).

  • 2. Place slides in slide racks and wash in a warm solution of Haemo-sol.

  • 3. Rinse slides well in H2O.

  • 4. Dip slides in the gelatin solution prepared in Step 1.

  • 5. Cover slides loosely and dry overnight.

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  1. doi:10.1101/pdb.rec12133 Cold Spring Harb Protoc 2010: pdb.rec12133-
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