DAB staining solution for Drosophila

0.5 mg/mL DAB (3,3′-diaminobenzidine tetrahydrochloride)

0.003% H₂O₂

1.5 mM CoCl₂

1.5 mM NiCl₂

The addition of CoCl₂ and NiCl₂ is optional. They intensify the reaction product and give it a blue-black color; in the absence of these compounds, the reaction product will be brown.

Soak all materials that become contaminated with DAB overnight in bleach prior to disposal.