Preparation of Late Drosophila Embryonic Fillets
Adapted from Drosophila Neurobiology (ed. Zhang et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2010.INTRODUCTION
Beyond the age of ~15 h (at 25°C), Drosophila embryos become impenetrable to aqueous fixatives and staining methods as a result of cuticle deposition. This problem can be overcome using flat or fillet preparations, as described in this protocol.
