Recipe

ATV solution (0.05%)

Reagent Amount to add (for 1 L)
NaCl 8 g
KCl 0.4 g
NaHCO3 0.58 g
Glucose 1.1 g
EDTA 0.2 g
Trypsin (2.5%) 20 mL
Dissolve the reagents in 500 mL of H2O. Adjust to a final volume of 1 L with H2O. Use HCl to adjust the pH to 7.4. Incubate the solution at 37°C for at least 1 h to activate the trypsin. Sterilize the solution through a 0.22-μm filter, prepare 50-mL aliquots, and store them at −20°C.
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  1. doi:10.1101/pdb.rec12326 Cold Spring Harb Protoc 2010: pdb.rec12326-
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