Recipe

Hyb

Reagent Quantity (for 50 mL) Final concentration
Formamide, deionized 25 mL 50%
Heparin 2.5 mg 50 μg/mL
SSC (20X) 12.5 mL 5X
Tween 20 50 μL 0.1%
Adjust the pH to 5.0 using HCl. Adjust the final volume to 50 mL with sterile H2O. Store at -20°C.
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  1. doi:10.1101/pdb.rec12332 Cold Spring Harb Protoc 2010: pdb.rec12332-
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