Recipe

Hyb-DNA-SDS

Reagent Quantity (for 15 mL) Final concentration
Hyb 14.85 mL
Salmon sperm DNA, sonicated (10 mg/mL; Invitrogen 15632-011) 150 μL 0.1 mg/mL
Store at -20°C.
Prior to use, warm the solution and add SDS (sodium dodecyl sulfate) to a final concentration of 0.3%.
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  1. doi:10.1101/pdb.rec12333 Cold Spring Harb Protoc 2010: pdb.rec12333-
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