Recipe

Histone demethylase dialysis buffer

Aprotinin (1 mg/mL)

DTT (dithiothreitol; 1 mM)

Glycerol (10%)

HEPES-KOH (40 mM, pH 7.9)

KCl (50 mM)

Leupeptin (1 mg/mL)

Pepstatin A (1 mg/mL)

PMSF (phenylmethylsulfonyl fluoride; 0.2 mM)

For Type II reactions, add 0.2 mM EDTA.

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  1. doi:10.1101/pdb.rec12334 Cold Spring Harb Protoc 2010: pdb.rec12334-
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