Recipe

Histone demethylase reaction buffer (type I)

α-ketoglutarate (1 mM)

Ascorbate (2 mM)

Ammonium ferrous sulfate (Fe[NH4]2[SO4]2; 7-700 μM)

HEPES-KOH (50 mM, pH 8.0)

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  1. doi:10.1101/pdb.rec12335 Cold Spring Harb Protoc 2010: pdb.rec12335-
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