Recipe

Histone storage buffer

Glycerol (10%)

HEPES-KOH (10 mM, pH 7.5)

KCl (10 mM)

PMSF (phenylmethylsulfonyl fluoride; 0.2 mM)

For Type II reactions, add 1 mM EDTA.

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  1. doi:10.1101/pdb.rec12337 Cold Spring Harb Protoc 2010: pdb.rec12337-
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