Recipe

SDS loading buffer (5X)

Bromophenol blue (0.25%)

DTT (dithiothreitol; 0.5 M)

Glycerol (50%)

SDS (sodium dodecyl sulfate; 10%)

Tris-Cl (0.25 M, pH 6.8)

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  1. doi:10.1101/pdb.rec12340 Cold Spring Harb Protoc 2010: pdb.rec12340-
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