DNA Extraction from Freeze-Dried Plant Tissue with CTAB in a 96-Well Format
Adapted from Genetic Variation (ed. Weiner et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2007.INTRODUCTION
This protocol is a modified version of DNA isolation using cetyltrimethylammonium bromide (CTAB) and 96-well plates. It is high-throughput, which facilitates the analysis of large mapping populations. The DNA yield is adequate for at least 100-500 polymerase chain reaction (PCR) procedures.
