Recipe

Array tomography blocking solution with BSA

Reagent Amount to add (for 1 mL) Final concentration
Bovine serum albumin (BSA), acetylated (10%; Electron Microscopy Sciences 25557)a 10 μL 0.1%
Tris-buffered saline (from tablets; Sigma-Aldrich T5030) 940 μL
Tween 20 (1%) (Electron Microscopy Sciences 25564)a 50 μL 0.05%
Prepare the day of use.
aThe 1% Tween and 10% BSA stocks can be stored for several months at 4°C.
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  1. doi:10.1101/pdb.rec12350 Cold Spring Harb Protoc 2010: pdb.rec12350-

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