Recipe

Protein digestion solution

25 mM ammonium bicarbonate (pH 8.0)

1 mM CaCl2

15 μg/mL trypsin

Aliquot the solution and store at -20°C. Minimize the number of freeze/thaw cycles (up to three times).

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  1. doi:10.1101/pdb.rec12363 Cold Spring Harb Protoc 2010: pdb.rec12363-
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