Microinjection of Xenopus Embryos
Adapted from Early Development of Xenopus laevis, by Hazel L. Sive, Robert M. Grainger, and Richard M. Harland. CSHL Press, Cold Spring Harbor, NY, USA, 2000.INTRODUCTION
This article describes the factors that should be considered when microinjecting Xenopus oocytes. Forward planning is particularly important for embryo injection because the frogs lay eggs only for a limited time, and once the eggs are fertilized there is a very short period during which the embryos are suitable for injection.
