Projection of a 3D time-lapse movie of Phallusia mamillata development from the 44-cell stage (stage 7 Hotta) to mid/late-gastrula stage (stage 12.5 Hotta)

Projection of a 3D time-lapse movie of Phallusia mamillata development from the 44-cell stage (stage 7 Hotta) to mid/late-gastrula stage (stage 12.5 Hotta) (Hotta K, Mitsuhara K, Takahashi H, Inaba K, Oka K, Gojobori T, Ikeo K. 2007. A web-based interactive developmental table for the ascidian Ciona intestinalis, including 3D real-image embryo reconstructions: I. From fertilized egg to hatching larva. Dev Dyn 236: 1790-805). The movie starts in animal view, rotates along the anteroposterior axis from the 44-cell stage to the early-110-cell stage, and subsequently shows gastrulation and blastopore closure. The movie spans >2 h, for 10 successive stacks. (For further reconstructions of time-lapse embryos and corresponding morphometric analysis, see Sherrard K, Robin F, Lemaire P, Munro E. 2010. Sequential activation of apical and basolateral contractility drives ascidian endoderm invagination. Curr Biol 20: 1499-1510.) [Supplemental movie from Imaging in Developmental Biology: A Laboratory Manual, Cold Spring Harbor Laboratory Press.]