Calibration of the Injection Volume for Microinjection of Xenopus Oocytes and Embryos
Adapted from Early Development of Xenopus laevis, by Hazel L. Sive, Robert M. Grainger, and Richard M. Harland. CSHL Press, Cold Spring Harbor, NY, USA, 2000.INTRODUCTION
Microinjection of Xenopus oocytes or embryos with messenger RNA (mRNA) or DNA is a powerful technique for studying development. Before microinjection can be performed, the injection volume must be calibrated carefully. This protocol describes the calibration procedure for a pressure injector.
