Recipe

Zero-calcium HL-3

Component Amount to add (for 1 L) Final concentration
NaCl 4.09 g 70 mM
MgCl2•6H2O 4.07 g 20 mM
NaHCO3 0.84 g 10 mM
Trehalose 1.89 g 5 mM
Sucrose 39.36 g 115 mM
HEPES 1.19 g 5 mM
KCl (1 M) 5 mL 5 mM
Na3EGTA (100 mM, pH 9.2) 5 mL 0.5 mM
1. Add all of the dry components to ~750 mL H2O. Stir to dissolve.
2. Add the KCl and Na3EGTA solutions.
3. Adjust pH to 7.2. Do not use KOH, because it will alter [K+] in the saline.
4. Adjust the final volume to 1 L with H2O.
| Table of Contents

This Article

  1. doi:10.1101/pdb.rec12365 Cold Spring Harb Protoc 2010: pdb.rec12365-
  1. » Full Text

Article Category

  1. Recipe

Personal Folder

  1. Save to Personal Folders

Updates/Comments

  1. Submit Updates/Comments
  2. No Updates/Comments published
  3. Alert me when Updates/Comments are published

Related Content

  1. Related Web Pages

Share

Current Issue

  1. January 2026, 2026 (1)
  1. Current Issue