Cover image

Cover Illustration: Image of adult zebrafish brain sections processed for in situ hybridization against early growth response factor 1 (egr I). Whole-mount brains were hybridized overnight with DIG-labelled antisense RNA, sectioned on a Vibratome, fixed on microscope slides, and treated for 4 h with BCIP/NBT. Images were taken with a Zeiss Axioplan 2 using a 10X objective. Stereoscopic picture of adult zebrafish brain is a composite of approximately 15 photographs assembled with Adobe Photoshop CS3. (Image courtesy of Enrico Kühn and Reinhard Köster.) For more information on this method, see Analysis of Gene Expression by In Situ Hybridization on Adult Zebrafish Brain Sections (doi:10.1101/pdb.prot5382).