HEPES-buffered saline (HEBS; 2X)
Dextrose (12 mM)
HEPES (50 mM)
KCl (10 mM)
NaCl (280 mM)
Na2HPO4•2H2O (1.5 mM)
Adjust the pH to 7.05 with 10 N NaOH; accurate pH is critical for efficient transfection. Sterilize by filtration through a 0.45-μm nitrocellulose filter. Store at -20°C.
