Recipe

Mono P enzyme solution

1 mg/mL pepsin

0.1 M acetic acid

0.5 M NaCl

Alternatively, try enzymes other than pepsin (e.g., DNase), depending on the type of contamination of the Mono P column.

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  1. doi:10.1101/pdb.rec12139 Cold Spring Harb Protoc 2010: pdb.rec12139-
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