Germination plates

Glufosinate ammonium (12 mg/L; Fluka 45520)

Kanamycin (50 mg/L)

MS medium for Arabidopsis (50%, v/v)

Phytagel (0.25%, w/v; Sigma-Aldrich P8169)

Prepare a Phytagel stock of 5 g/L in H₂O. Autoclave. Cool to 60°C. Mix the Phytagel stock with an equal volume of MS medium (i.e., to the final concentrations listed above). Add the kanamycin and glufosinate ammonium. Working under a hood, pour into Petri dishes (~25 mL per 100-mm plate).