Recipe

Hybridization buffer for adult zebrafish

Reagent Amount to add Final concentration
Formamide (100%) 25 mL 50%
Heparin (50 mg/mL) 50 μL 50 μg/mL
SSC (20X) 12.5 mL 5X
Torula RNA 250 mg 5 mg/mL
Tween 20 (10%) 500 μL 0.1%
H2O to 50 mL --
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  1. doi:10.1101/pdb.rec12145 Cold Spring Harb Protoc 2010: pdb.rec12145-
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