Recipe

NEB buffer 2 for washes (1X)

50 mM NaCl

10 mM Tris-Cl

10 mM MgCl2

1 mM DTT (dithiothreitol)

Adjust pH to 7.9.

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  1. doi:10.1101/pdb.rec12150 Cold Spring Harb Protoc 2010: pdb.rec12150-
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