BrdU (100X)

BrdU (5-bromo-2′-deoxyuridine; Sigma B5002)

10 mM Tris-Cl (pH 7.6)

For a 100X stock solution, prepare BrdU at 40 mg/mL in 10 mM Tris-Cl (pH 7.6) in a 15-mL screw-cap conical tube. Place the solution in a waterbath at ~50ºC and occasionally vortex vigorously until the BrdU is completely dissolved. Store the solution in convenient (e.g., single-use) aliquots protected from light at -20°C. Upon thawing, BrdU may come out of solution and need to be redissolved.