Table

Table 3. Polymeric stationary phases for chromatofocusing

Chromatofocusing matrix/column Matrix composition Ligands Maximum loading capacity pH working range Column dimensions Recommended flow rate Maximum flow rate
PBE 94 Sepharose 6B Tertiary and quaternary amines 20 mg of protein/mL
of matrix 		4-9 bulk 30-40 cm/h 75 cm/h
PBE 118 Sepharose 6B Tertiary and quaternary amines 20 mg of protein/mL
of matrix 		8-11 bulk 30-40 cm/h 75 cm/h
Mono P 5/5 Polystyrene-divinyl benzene Tertiary and quaternary amines 10 mg of protein 4-11 5 × 50 mm 0.5-1.5 mL/min 1.5 mL/min
Mono P 5/20 Polystyrene-divinyl benzene Tertiary and quaternary amines 40 mg of protein 4-11 5 × 200 mm 0.5-1.5 mL/min 1.5 mL/min
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  1. doi:10.1101/pdb.tab395379 Cold Spring Harb Protoc 2010: pdb.tab395379-
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