Chromatofocusing
Adapted from Purifying Proteins for Proteomics: A Laboratory Manual (ed. Simpson). CSHL Press, Cold Spring Harbor, NY, USA, 2004.INTRODUCTION
Chromatofocusing separates proteins on the basis of differences in their isoelectric point (pI). The stationary phase (matrix) is usually a weak anion exchanger in which the functional groups are amines, for example, a polybuffer exchanger (PBE 118 and PBE 94) or Mono P column. The eluent is a buffer containing a large number of buffering species that together give a uniform buffering capacity over a broad pH range.
