Recipe

MEM for neuron culture

Minimal Essential Medium (MEM; contains Earle’s salts) (GIBCO/Invitrogen 11095-080)

0.001% ovalbumin (Sigma)

25 ng/mL insulin (Sigma)

1 mg/mL transferrin (Sigma)

10 mM sodium pyruvate (Sigma)

2 ppm progesterone (Sigma)

3 ppm selenium dioxide (SeO2) (Sigma)

0.5 nM putrescine (Sigma)

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  1. doi:10.1101/pdb.rec12181 Cold Spring Harb Protoc 2010: pdb.rec12181-
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