Acrylamide stock (40% T, 2.67% C)
Add acrylamide and bisacrylamide to 100 mL of H2O. Filter the stock solution using Whatman filter paper and store at 4°C. Prepare fresh acrylamide stock solution every few weeks.
T denotes the total percentage concentration of the monomers (acrylamide plus N,N′-methylenebisacrylamide) in grams per 100 mL. C denotes the percentage (by weight) of the cross-linker N,N′-methylenebisacrylamide relative to the total amount of monomers.
