Recipe

HBS solution for virus production (2X)

280 mM NaCl

50 mM HEPES

1.42 mM Na2HPO4•7H2O (pH 7.05)

Adjust pH to 7.05 with 10 M NaOH.

Sterilize by passing through a 0.22-μm filter. Store at room temperature.

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  1. doi:10.1101/pdb.rec12198 Cold Spring Harb Protoc 2010: pdb.rec12198-
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