Recipe

X-gal solution for brain

0.1 M phosphate buffer for mouse brain

2 mM MgCl2

0.01% sodium deoxycholate

0.02% Nonidet P-40 (NP-40)

10 mM K3Fe(CN)6 (potassium ferricyanide)

10 mM K4Fe(CN)6•3H2O (potassium ferrocyanide)

0.001% X-gal

IGEPAL CA-630 can be used in place of NP-40.

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  1. doi:10.1101/pdb.rec12206 Cold Spring Harb Protoc 2010: pdb.rec12206-
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