|
|
| Uniform acrylamide concentration gels |
|
| 5 |
2.6 |
25,000-300,000 |
| 10 |
2.6 |
15,000-100,000 |
| 10 |
3.0b |
1,000-100,000 |
| 15 |
2.6 |
12,000-50,000 |
|
|
| Gradient acrylamide gelsc |
|
| 3.3-12 |
2.0d |
14,500-2,800,000 |
| 3-30 |
8.4 |
13,000-1,000,000 |
| 5-20 |
2.6 |
14,000-210,000 |
| 8-15 |
1.0 |
14,000-330,000 |
|
| Reproduced, with permission, from Shi and Jackowski (1998). |
| aT denotes the total percentage concentration of the monomers (acrylamide plus N,N′-methylenebisacrylamide) in grams per 100 mL. C denotes the percentage (by weight) of the cross-linker N,N′-methylenebisacrylamide relative to the total amount of monomers.
|
| bWhen using tricine instead of glycine in running buffer (Schägger and von Jagow 1987).
|
| cThis list is by no means exhaustive and other gel ranges may also be used.
|
| dGranzier and Wang (1993).
|
