Table 2. Resolving gel recipes

% T^b	Acrylamide stock (30.0%) (mL)	Resolving gel buffer (mL)	H₂O (mL)	10% SDS (mL)	10% Ammonium persulfate^c (mL)	TEMED^d (mL)
For making 100 mL of resolving gel mixture^a
8.0	26.67	25.0	46.53	1.0	0.75	0.05
9.0	30.00	25.0	43.20	1.0	0.75	0.05
10.0	33.33	25.0	39.87	1.0	0.75	0.05
11.0	36.67	25.0	36.53	1.0	0.75	0.05
12.0	40.00	25.0	33.20	1.0	0.75	0.05
13.0	43.33	25.0	29.87	1.0	0.75	0.05
14.0	46.67	25.0	26.53	1.0	0.75	0.05
15.0	50.00	25.0	23.20	1.0	0.75	0.05
16.0	53.33	25.0	19.87	1.0	0.75	0.05
17.0	56.67	25.0	16.53	1.0	0.75	0.05
18.0	60.00	25.0	13.20	1.0	0.75	0.05
19.0	63.33	25.0	9.87	1.0	0.75	0.05
20.0	66.67	25.0	6.53	1.0	0.75	0.05

^aThis is enough for four 1.0-mm thick Bio-Rad Protean II xi gels (160 × 150 × 1 mm³).
^bT denotes the total percentage concentration of the monomers (acrylamide plus N,N′-methylenebisacrylamide) in grams per 100 mL.
^cThe 10% ammonium persulfate solution must be made fresh.
^dAdd TEMED just before pouring the gel.