Table

Table 2. Key examples of live cell imaging of different Drosophila tissues

Tissue type Subject Approacha References
Various tissues Chromosomes/division/nuclei nls-GFP, histone-GFP, H2Av-mRFP1, EGFP-Cid (kinetochores) Davis et al. 1995; Yucel et al. 2000; Pauli et al. 2008
For numerous cell components Numerous stains and dyes Molecular Probes (see “Web Resources”)
Egg chambers Border cell migration GFP fluorescence Tekotte et al. 2007; McDonald et al. 2008
Golgi units GalT, a GFP-tagged line Morin et al. 2001; Nicolas et al. 2009
Microtubules τ-GFP, tubulin-GFP, Jupiter-GFP Endow and Komma 1997; Micklem et al. 1997; Grieder et al. 2000; Karpova et al. 2006
mRNA transport MS2/MCP-RFP and GFP, Alexa Fluor 488/546-RN MacDougall et al. 2003; Clark et al. 2007b; Weil et al. 2008; Zimyanin et al. 2008
Testes Spermatogenesis, cell division GFP, FRAP Noguchi and Miller 2003
Gonads Gonadal cell migration Six4-nls-eGFP Clark et al. 2007a
Embryos Lipid droplet motility DIC Welte et al. 1998
Movement of Wg signal Wg-GFP Pfeiffer et al. 2002
Following the movement of individual chromosome sites LacI-LacO-GFP Marshall et al. 1997
General labeling of embryos Feeding adults rhodamine B I Davis (unpubl.)
Asymmetric cell division τ-GFP Kaltschmidt et al. 2000
FRAP Llg-GFP Wirtz-Peitz et al. 2008
Macrophage migration, wound healing Cytoplasmic GFP Stramer et al. 2005
Apoptosis, neuronal cell ablation Caspase sensor (Apoliner), GFP Hidalgo and Brand 1997; Bardet et al. 2008
Marking clones Caged-FITC-dextran Vincent and O’Farrell 1992
mRNA transport Alexa Fluor 546-RNA Bullock and Ish-Horowicz 2001; Wilkie and Davis 2001; Vendra et al. 2007
Larvae Salivary gland chromosomes DIC, GFP Vazquez et al. 2001, 2002
Imaginal discs Argos-GFP, Dpp-GFP Entchev et al. 2000; Greco et al. 2001
Mitochondria transport Mito-GFP Pilling et al. 2006
Brain neuroblast cell cycle GFP trap line, centrosomin-GFP Rusan and Peifer 2007; Siller and Doe 2008
Synapse addition GFP/RFP/FM dyes, FRAP-GFP Rasse et al. 2005; Schmid et al. 2008
Dendrite morphogenesis Rab5-GFP, 2xFYVE-GFP Satoh et al. 2008
PNS EB1-GFP, mCD8-GFP Rolls et al. 2007
NMJs Calcium sensor Reiff et al. 2005
NMJs, vesicle trafficking FM1-43 Verstreken et al. 2008
Pupae Glial migration in wing disc UV laser ablation Gho et al. 1999; Aigouy et al. 2008
Asymmetric cell division GFP
Adults Eye in whole adult Rhodopsin Mollereau et al. 2000; Pichaud and Desplan 2001
Brain Calcium and camgaroo, Cameleon calcium sensor Yu et al. 2003; Riemensperger et al. 2005; Hendel et al. 2008
aAbbreviations: DIC: differential interference contrast; eGFP: enhanced green fluorescent protein; FITC: fluorescein isothiocyanate; FRAP: fluorescence recovery after photobleaching; GFP: green fluorescent protein; NMJs: neuromuscular junctions; PNS: peripheral nervous system; RFP: red fluorescent protein; UV: ultraviolet.
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  1. doi:10.1101/pdb.tab2top75 Cold Spring Harb Protoc 2010: pdb.tab2top75-

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