| Dry |
20X 0.75-NA, 40X 0.95-NA, 50X 0.5-NA LMPLFLN |
#1.5-coverslip-corrected, long working distance |
Deep imaging in thick tissues (e.g., embryos); micromanipulation and injection (upright microscope) |
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| WI |
60X 1.2-NA WI |
Coverslip correction collar, sensitive to coverslip angle, good working distance |
Deep imaging in thick tissues (e.g., embryos); multiphoton imaging |
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| Water dipping |
60X 0.9-NA, 100X 1.0-NA LUMPFL |
Upright microscopes, no coverslip, long working distance |
High-resolution imaging of live material in an upright configuration |
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| Multi-immersion (glycerol) |
20X 0.75-NA, 60X 1.3-NA |
80% glycerol (better matched to RI of cytoplasm), coverslip correction collar, sensitive to coverslip angle |
Deep imaging in thick tissues (e.g., embryos); multiphoton imaging |
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| Silicone oil |
60X 1.3-NA silicone |
Requires silicone oil (better matched to RI of cytoplasm), coverslip correction collar, sensitive to coverslip angle |
Improved deep imaging in thick tissues (e.g., embryos) |
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| Conventional oil |
40X 1.3-NA to 100X 1.4-NA |
#1.5-coverslip-corrected, use a range of oils of varying RI to correct for spherical aberration |
Tissue-culture cells; imaging within maximum 30 μm of the coverslip; high-resolution imaging; fixed material |
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| aExamples refer to Olympus objectives; in most cases, similar objectives are available from other manufacturers.
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| bAbbreviations: NA: numerical aperture; RI: refractive index; WI: water immersion.
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