Protocol

DNA Isolation from Rat Tail or Ear

Adapted from Genetic Variation (ed. Weiner et al.). CSHL Press, Cold Spring Harbor, NY, USA, 2007.

INTRODUCTION

This protocol describes a rapid procedure for isolating DNA from rat tail or ear punches. The simplest version of the protocol can be scaled for use in 96-well (deep-well) plates. The quality of the DNA is sufficient for any polymerase chain reaction (PCR)-based genotyping approach.

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