Recipe

Cocultivation medium

1.13 g/L Murashige and Skoog medium (Caisson Laboratories)

1% sucrose

100 μM acetosyringone

0.005% (v/v; 50 μL/L) Silwet L-77

Adjust pH to 6.0 with KOH.

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  1. doi:10.1101/pdb.rec12213 Cold Spring Harb Protoc 2010: pdb.rec12213-
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