CM selective media

Ingredients	Glu/CM −U−H medium	Glu/CM −U−H agar	Glu/CM −T agar	Glu/CM −U−H−T agar	Glu/CM −U−H−T−L agar	Gal/Raf −U−H agar	Gal/Raf/CM −U−H−L agar	Gal/Raf/CM −U−H−T medium	Gal/Raf/CM −U−H−T agar	Gal/Raf/CM −U−H−T−L agar
YNB	6.7 g	6.7 g	6.7 g	6.7 g	6.7 g	6.7 g	6.7 g	6.7 g	6.7 g	6.7 g
Glucose	20 g	20 g	20 g	20 g	20 g	--	--	--	--	--
Galactose + raffinose	--	--	--	--	--	20 g + 10 g	20 g + 10 g	20 g + 10 g	20 g + 10 g	20 g + 10 g
Dropout mix	2 g	2 g	2 g	2 g	2 g	2 g	2 g	2 g	2 g	2 g
Leucine (L) (4 mg/mL)	15 mL	15 mL	15 mL	15 mL	--	15 mL	--	15 mL	15 mL	--
Histidine (H) (4 mg/mL)	--	--	5 mL	--	--	--	--	--	--	--
Tryptophan (T) (4 mg/mL)	10 mL	10 mL	--	--	--	10 mL	10 mL	--	--	--
Uracil (U) (4 mg/mL)	--	--	5 mL	--	--	--	--	--	--	--
Agar	--	20 g	--	20 g	20 g	20 g	20 g	--	20 g	20 g

To prepare medium or agar, mix the ingredients together in a final volume of 1 L of H₂O. Autoclave for 20 min. Cool the medium to 50ºC before pouring plates (24 × 24 cm).
Yeast nitrogen base without amino acids (YNB) is sold either with or without ammonium sulfate. This recipe is for YNB with ammonium sulfate. If the bottle of YNB is lacking ammonium sulfate, add 5 g of ammonium sulfate and only 1.7 g of YNB.
Adapted from Sambrook and Russell (Sambrook J, Russell D. 2001. Molecular cloning: A laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.).