Growth medium (pH 7.4) (DMEM-10)
Dulbecco’s modified Eagle’s medium (DMEM; GIBCO/Invitrogen)
10% normal calf serum
1% penicillin/streptomycin (Cellgro)
1% L-glutamine (Cellgro)
Filter-sterilize through a 0.22-μm filter.
Dulbecco’s modified Eagle’s medium (DMEM; GIBCO/Invitrogen)
10% normal calf serum
1% penicillin/streptomycin (Cellgro)
1% L-glutamine (Cellgro)
Filter-sterilize through a 0.22-μm filter.